アカイ ショウジ   Akai Shoji
  赤井 昭二
   所属   栄養学部
   職種   教授
言語種別 英語
発行・発表の年月 2003/06
形態種別 研究論文
査読 査読あり
標題 Chemoenzymatic synthesis of [3,9-13C]-labeled NeuAc and KDN
執筆形態 共著
掲載誌名 Tetrahedron Lett.
掲載区分国外
出版社・発行元 Elsevier
巻・号・頁 44(17),pp.3513-3516
著者・共著者 K. Sato, S. Akai, T. Hiroshima, H. Aoki, M. Sakuma K. Suzuki
概要 The chemoenzymic synthesis of 13C-labeled sialic acid (NeuAc) and 3-deoxy-D-glycero-D-galacto-2-nonulosonic acid (KDN) as useful mol. probes for studying the conformation of sialyl or KDN oligosaccharides attached to proteins was performed by using [6-13C]-ManNAc, [6-13C]-Man and [3-13C]-pyruvic acid sodium salt. In the synthesis of the compds., 5,6-anhydro intermediates were found to easily provide not only 6-13C-labeled but also 5-, and 6-modified NeuAc and KDN analogs. Furthermore, it was demonstrated that identical results are obtained by NMR for both [3,9-13C]-NeuAc and 1:1 mixts. of [3-13C]- and [9-13C]-NeuAc.