ウスイ　テルユキ   Teruyuki USUI
  臼井　照幸
   所属   栄養学部
   職種   教授
言語種別 英語
発行・発表の年月 2018/05
形態種別 研究論文
査読 査読あり
標題 Identification of pyridinoline, a collagen crosslink, as a novel intrinsic ligand for the receptor for advanced glycation end-products (RAGE)
執筆形態 共著
掲載誌名 Bioscience, Biotechnology, and Biochemistry
掲載区分国内
巻・号・頁 pp.1508-1514
著者・共著者 Yoto Murakami, Takayuki Fujino, Ryotaro Kurachi, Toshiki Hasegawa, Teruyuki Usui, Fumitaka Hayase & Hirohito Watanabe
概要 We hypothesized that pyridinoline (Pyr), a collagen crosslink containing the 3-HP moiety, could have agonist activity with RAGE. To test this hypothesis, we purified Pyr from bovine achilles tendons and examined its cytotoxicity to rat neuronal PC12 cells. Pyr elicited toxicity to PC12 cells in a concentration-dependent manner, and this effect was attenuated in the presence of either the anti-RAGE antibody or the soluble form of RAGE. Moreover, surface plasmon resonance-based analysis showed specific binding of Pyr to RAGE. These data indicate that Pyr is an intrinsic ligand for RAGE.