ウスイ テルユキ   Teruyuki USUI
  臼井 照幸
   所属   栄養学部
   職種   教授
言語種別 英語
発行・発表の年月 2018/01
形態種別 研究論文
査読 査読あり
標題 Receptor for advanced glycation end products (RAGE)-mediated cytotoxicity of 3-hydroxypyridinium derivatives
執筆形態 共著
掲載区分国外
出版社・発行元 Bioscience, Biotechnology, and Biochemistry
著者・共著者 Yoto Murakami, Takayuki Fujino, Toshiki Hasegawa, Ryotaro Kurachi, Aya Miura, Takumi Daikoh, Teruyuki Usui, Fumitaka Hayase, Hirohito Watanabe
概要 Advanced glycation end products (AGEs) formed from glyceraldehyde (Gcer) and glycolaldehyde (Gcol) are involved in the pathogenesis of diabetic complications, via interactions with a receptor for AGEs (RAGE). In this study, we aimed to elucidate the RAGE-binding structure in Gcer and Gcol-derived AGEs and identify the minimal moiety recognized by RAGE. Among Gcer and Gcol-derived AGEs, GLAP (glyceraldehyde-derived pyridinium) and GA-pyridine elicited toxicity in PC12 neuronal cells. The toxic effects of GLAP and GA-pyridine were suppressed in the presence of anti-RAGE antibody or the soluble form of RAGE protein. Furthermore, the cytotoxicity test using GLAP analog compounds indicated that the 3-hydroxypyridinium (3-HP) structure is sufficient for RAGE-dependent toxicity. Surface plasmon resonance analysis showed that 3-HP derivatives directly interact with RAGE.