ヒライシ サユリ   
  平石 さゆり
   所属   栄養科学研究所
   職種   専任講師
言語種別 英語
発行・発表の年月 1998
形態種別 研究論文(学術雑誌)
査読 査読あり
標題 Characterization of soluble thrombomodulin fragments in human urine.
執筆形態 共著
掲載誌名 Thromb. Haemost.
巻・号・頁 79,pp.331-337
総ページ数 null
著者・共著者 Nakano M, Furutani M, Hiraishi S, Ishii H
概要 The soluble thrombomodulin (TM) subspecies in human urine detected by polyclonal anti-human TM IgG were isolated and characterized. 105, 85, 80, 56, 33, 31 and 28 kDa subspecies under reducing conditions was comparable to 78, 66, 56, 200, 52, 30 and 25 kDa under non-reducing conditions, respectively, in the two-dimensional electrophoresis. NH2-terminal amino acid sequences of the 105, 85 and 80 kDa subspecies maintained Ala1-Pro2-Ala3- of intact human TM. All subspecies obtained under non-reducing conditions exhibited cofactor activity for thrombin-dependent protein C activation indicating that all of the subspecies maintained the fourth to sixth repeat of epidermal growth factor-like structure of intact TM. 85, 80, 56, 33, 31 and 28 kDa subspecies were suggested to lack both chondroitin sulfate glycosaminoglycan (CSGAG), transmembrane and cytoplasmic domains of intact TM, while 105 kDa subspecies lack only CSGAG.