ヒライシ サユリ   
  平石 さゆり
   所属   栄養科学研究所
   職種   専任講師
言語種別 英語
発行・発表の年月 2002
形態種別 学位論文(博士)
査読 査読あり
標題 Oxidized low-density lipoprotein associates strongly with carboxy-terminal domain of tissue factor pathway inhibitor and reduces the catalytic activity of the protein.
執筆形態 共著
掲載誌名 Thromb. Haemost.
巻・号・頁 87,pp.80-85
総ページ数 null
著者・共著者 Horie S, Hiraishi S, Hamuro T, Kamikubo Y, Matsuda J
概要 Previously we have shown that tissue factor pathway inhibitor (TFPI) associates quite rapidly with oxidized low-density lipoprotein (ox-LDL), with a reduction of the inhibitory activity on factor X activation. In the present study, it was found, by means of agarose gel electrophoresis, that the pre-incubation of full-length rTFPI with heparin or the carboxy (C)-terminal part (peptide 240-265) of TFPI prevented the association with ox-LDL in a dose-dependent manner. When rTFPI lacking the C-terminal basic part of the molecule (rTFPI-C) was mixed with ox-LDL, only a small amount of rTFPI-C was shifted to the position of ox-LDL on electrophoresis. Further, ox-LDL did not reduce the activity of rTFPI-C. These results indicate that the C-terminal domain of TFPI molecule plays a predominant role in the binding to ox-LDL and the binding through the C-terminal part is essential for the ox-LDL-dependent reduction of the anticoagulant activity of TFPI.