ヒライシ サユリ   
  平石 さゆり
   所属   栄養科学研究所
   職種   専任講師
言語種別 英語
発行・発表の年月 1990
形態種別 研究論文(学術雑誌)
標題 Establishment of enzyme immunoassay of human thrombomodulin in plasma and urine using monoclonal antibodies.
執筆形態 共著
掲載誌名 Thromb. Hemost.
巻・号・頁 63(2),pp.157-162
総ページ数 null
著者・共著者 Ishii H, Nakano M, Tsubouchi J, Ishikawa T, Uchiyama H, Hiraishi S, Tahara C, Miyajima Y, Kazama M
概要 Thrombomodulin, TM, is an endothelial cell membrane protein acting as a cofactor for the activation of plasma protein C. Soluble TM is present in plasma and urine of normal subjects. Enzyme immunoassay, EIA, for human TM was developed using mouse monoclonal antibodies against human placental TM in this paper. EIA sandwich method using three types of the monoclonal antibodies enabled us to measure almost all of 6 and 7 TM subspecies in plasma and urine, respectively, except 1 subspecies, 31 kDa TM. There was no interference from other components of plasma and urine in the assay conditions. TM levels in plasma and urine of normal subjects collected in the morning were 35.2 +/- 8.32 ng/ml (n = 346) and 111 +/- 31.6 ng/ml (n = 33), respectively. TM level in plasma did not differ from the level in serum. Circadian fluctuation of plasma TM was not significant in 10 normal adults, although a tendency of increase in TM excretion to urine was found rather in the day time than the other times.