ヒライシ サユリ   
  平石 さゆり
   所属   栄養科学研究所
   職種   専任講師
言語種別 英語
発行・発表の年月 1990
形態種別 研究論文(学術雑誌)
査読 査読あり
標題 Thiolprotease inhibitor, EST, can inhibit thrombin-induced platelet activation.
執筆形態 共著
掲載誌名 Thromb. Res.
巻・号・頁 57,pp.847-861
総ページ数 null
著者・共著者 Ishii H, Kuboki M, Fujii J, Hiraishi S, Kazama M
概要 Participation of thiolprotease in platelet activation was investigated. When platelets were treated with EST (L-trans-epoxysuccinyl-leucylamide (3-methyl)butane-ethyl ester, a membrane-permeable thiolprotease inhibitor), thrombin or platelet activating factor (PAF) -induced aggregation and secretion were inhibited, and remained so even when the platelets were washed and resuspended in EST-free medium after the pretreatment. Pretreatment of platelets with EST inhibited the calpain (thiolprotease) activity in platelets. The phosphorylation of 40 kDa and 20 kDa proteins of platelets caused by stimulation with thrombin was blocked by the pretreatment with 1 mM EST. Phosphatidylinositol hydrolysis and inositol-1-phosphate production, which appear after stimulation of platelets with thrombin, were also inhibited by the pretreatment with 1 mM EST. The results suggest that EST was incorporated to inside of platelets, and inhibited activation of platelet through inhibition of thiolprotease.